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g6501 protoscriptii cdna synthesis kit neb  (New England Biolabs)


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    New England Biolabs g6501 protoscriptii cdna synthesis kit neb
    G6501 Protoscriptii Cdna Synthesis Kit Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g6501+protoscriptii+cdna+synthesis+kit+neb/ProtoScript+II+Reverse+Transcriptase/pm41785860-337-276-281
    Average 98 stars, based on 1434 article reviews
    g6501 protoscriptii cdna synthesis kit neb - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Bradford Assay:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Staining:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Stripping Membranes:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Recombinant:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Plasmid Preparation:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Labeling:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Antibody Labeling:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Ligation:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    cDNA Synthesis:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2

    Real-time Polymerase Chain Reaction:

    Article Title: Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Putrescine Sigma Cat# P5780 bFGF R&D Systems Cat# 3718-FB Phenylmethanesulfonyl fluoride (PMSF) Sigma Cat# 93482 NP-40 Sigma Cat# I8896 Protease Inhibitor Cocktail Sigma Cat# P1860 PierceTM Bradford Assay Reagent Thermo Scientific Cat# 23238 4x Laemmli Sample Buffer Bio-Rad Cat# 1610747 Trichloroacetic acid (TCA) Sigma Cat# T6399 Goat Serum Sigma Cat# G9023 Hoechst stain Sigma Cat# 94403 ProLong® Gold Antifade reagent Invitrogen Cat# P10144 Radiance Plus chemiluminescent HRP substrate Azure Cat# AC2103 NewBlotTM PVDF Stripping Buffer LI-COR Cat# 928-40032 Lysozyme Sigma Cat# L6876 RNase A ThermoFisher Scientific Cat# 12091021 Protease inhibitor tablet Sigma Cat# 5892970001 Poly(ethyleneimine) (PEI) Sigma Cat# P3143 PierceTM Glutathione Agarose Thermo Scientific Cat# 16100 ANTI-FLAG-M2 affinity resin Sigma Cat# A2220 FLAG peptide Sigma Cat# F3290 PierceTM Detergent Compatible Bradford Assay Reagent Thermo Scientific Cat# 1863028 PreScission Protease Cytiva Cat# 27-0843-01 Recombinant PRC2 complex Active Motif Cat# 31387 HaloTag® Standard Protein Promega Cat# G4491 FluoroBrite DMEM Life Technologies Cat# A1896701 Janelia Fluor® 549 HaloTag® Ligand Promega Cat# GA1110 L-Glutathione Sigma Cat# G4251 dTag-13 Tocris Cat# 6605 Mononucleosome EpiCypher Cat# 16-2025 11-mer Polynucleosome Active Motif Cat# 31466 Tetra-Nucleosome This Study N/A Uranyl Formate Fisher Scientific Cat# 16984-59-1 Critical commercial assays DNEasy Blood and Tissue Kit QIAGEN Cat# 69504 RNEasy Mini Kit QIAGEN Cat# 74104 EndoFree Maxi Plasmid Kit QIAGEN Cat# 12362 Midi Plasmid Kit QIAGEN Cat# 12143 Protein Labeling Kit (Alexa Fluor® 555) Thermo Scientific Cat# A20174 Antibody Labeling Kit (Alexa Fluor® 647) Thermo Scientific Cat# A20186 CUTANATM ChIC/CUT&RUN Kit EpiCypher Cat# 14-1048 CUTANATM CUT&Tag Kit EpiCypher Cat# 14-1102 Dovetail® Proximity Ligation Core Box 1 Cantata-Bio Cat# DG-REF-001 Dovetail® Micro-C Module Box 2 Cantata-Bio Cat# DG-NUC-001 HaloTag® Mammalian Pull-Down Promega Cat# G6501 ProtoScriptII cDNA Synthesis Kit NEB Cat# M0368S StepOneTM Real-Time PCR System Applied Biosystems N/A NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® NEB Cat# E7760S (Continued on next page) Molecular Cell 86, 1–17.e1–e16, March 19, 2026 e2



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